Cypress CSC-5300 Manual do Utilizador descarregar pdf (Página 73)

66 Chapter 6: Sample Experiments

Calorimetry Sciences Corp.

CSC 5300 N-ITC III 67

User’s Manual

the tip of the injection needle during equilibration, which results in fewer moles of titrant

being injected in the rst injection. If you want to discard this point in the t then position

the mouse pointer directly over the point and click the left button once. The point should

be replaced by a red triangle, indicating that the integrated area will not be used in the t.

Next, click the Fit Selected Model button. The values for K, ∆H and n will be displayed

in a table located directly above a graph showing both the data and t. If you would like

statistical analysis of the t click the Statistical Analysis button.

Figure 6-6: Modeling the RNase A experiment.

Statistical analysis

Fit selected

model

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