Cypress CSC-5300 Manual do Utilizador Página 1

Nano-Isothermal Titration Calorimeter IIIModel CSC 5300User’s Manual

4 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 5 User’s Manualserver) and one person us

4 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 5 User’s Manualor other use expressly pe

6 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 7 User’s ManualThis page intentionally l

6 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 7 User’s ManualChapter Two: Installation

8 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 9 User’s Manualorder to obtain optimum performance from

8 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 9 User’s ManualProduct Items• Model 5300 Nano-Isotherm

10 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 11 User’s Manual3. Plug the loose USB cable into the

10 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 11 User’s Manual5300 icon located on the desktop or by

12 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 13 User’s ManualThis page intentionally left blank.

12 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 13 User’s ManualChapter Three: Theory of OperationIntr

CSC 5300 N-ITC III i User’s ManualIsothermal Titration Calorimeter, User’s Manual Revision 40605Copyright ©2005 Calorimetry Sciences

14 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 15 User’s Manualused to determine titrate conce

14 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 15 User’s ManualFigure 3-3: Baseline heat outli

16 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 17 User’s ManualTitration/Data AnalysisA single

16 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 17 User’s Manualshows that increased overall cu

18 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 19 User’s ManualIn order to determine binding c

18 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 19 User’s Manualwhich can then be substituted i

20 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 21 User’s Manualsite per protein based on struc

20 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 21 User’s ManualFreire, E., Mayorga, O. L. &

22 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 23 User’s ManualThis page intentionally left bl

22 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 23 User’s ManualChapter Four: Hardware Descript

CSC 5300 N-ITC III i User’s ManualTable of ContentsCHAPTER ONE: SPECIFICATIONS AND WARRANTY. 1Calorimeter Specications ...

24 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 25 User’s ManualFigure 4-1). The power require

24 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 25 User’s ManualExtreme care should be taken n

26 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 27 User’s Manualrotating shaft of the burette

26 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 27 User’s ManualFigure 4-6: Relative positions

28 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 29 User’s Manualthe calorimeter is the reverse

28 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 29 User’s ManualPower SupplyThe calorimeter ha

30 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 31 User’s Manualadapter into the sample cell,

30 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 31 User’s Manual5. Apply a vacuum to draw the

32 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 33 User’s ManualThis page intentionally left b

32 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 33 User’s ManualChapter Five: Software Descrip

ii Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III iii User’s ManualReinstalling Software...

34 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 35 User’s ManualITCRun will initialize and the

34 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 35 User’s Manualdata le for review. Also appe

36 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 37 User’s Manualcommands are described below.E

36 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 37 User’s ManualThe actual injection rate in t

38 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 39 User’s ManualBuret • Move DownThe Buret Mov

38 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 39 User’s Manualbe used. Click on the stirrer

40 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 41 User’s ManualSetup ButtonUsed to enter the

40 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 41 User’s ManualReset chartThis option complet

42 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 43 User’s Manualon the data tab my be enlarged

42 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 43 User’s Manualslowly to allow air bubbles to

ii Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III iii User’s ManualCHAPTER FIVE: SOFTWARE DESCRIPTION ...

44 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 45 User’s Manualthen screw the syringe complet

44 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 45 User’s ManualProgram indicator in ITCRun wi

46 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 47 User’s ManualGetting StartedInstalling Bind

46 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 47 User’s Manual2. Select File⇒Open from the

48 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 49 User’s Manual Figure 5-22: Viewing the area

48 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 49 User’s ManualAs you change the baseline or

50 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 51 User’s ManualThe initial Area display is sh

50 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 51 User’s ManualModelingModeling OverviewEach

52 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 53 User’s ManualV - is the volume of the cell

52 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 53 User’s ManualRectangular regions represent

iv Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III 1 User’s ManualIntegration of the peak ...

54 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 55 User’s ManualCooperative Binding Model This

54 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 55 User’s ManualWhere Xb is the amount of liga

56 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 57 User’s ManualIn an experiment designed to m

56 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 57 User’s ManualEnter 1 mM for the ligand conc

58 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 59 User’s ManualThis page intentionally left b

58 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 59 User’s ManualChapter Six: Sample Experiment

60 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 61 User’s ManualExperiment Walk-ThroughIntroduct

60 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 61 User’s ManualFigure 6-1: Filling the N-ITC II

62 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 63 User’s Manualand click the burette up icon to

62 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 63 User’s Manualwhere t is given in °C†.† Christ

iv Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III 1 User’s ManualChapter One: Specications and WarrantyCalori

64 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 65 User’s ManualThe presence of bubbles in the s

64 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 65 User’s ManualBlank TitrationThe heat effect m

66 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 67 User’s Manualprogram main screen (Figure 6-7)

66 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 67 User’s Manualthe tip of the injection needle

68 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 69 User’s ManualThis page intentionally left bla

68 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 69 User’s ManualChapter Seven: TroubleshootingMi

70 Chapter 7: Troubleshooting Calorimetry Sciences Corp.This page intentionally left blank.

2 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 3 User’s Manual• Unauthorized modicati

2 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 3 User’s ManualThis limited warranty giv