Cypress CSC-5300 Manual do Utilizador descarregar pdf (Página 6)

iv Table of Contents

Calorimetry Sciences Corp.

CSC 5300 N-ITC III 1

User’s Manual

Integration of the peak .........................................................49

Modeling and Fit of the Data...............................................50

Modeling .................................................................................51

Modeling Overview .............................................................51

Designing an Experiment ......................................................55

CHAPTER SIX: SAMPLE EXPERIMENTS ................... 59

Experiment Overview............................................................59

Experiment Walk-Through...................................................60

Introduction............................................................................60

Chemical Calibration.............................................................60

Heat of Protonation of Tris Base..........................................60

Sample Experiment................................................................63

Binding of 2’-CMP to RNase A...........................................63

CHAPTER SEVEN: TROUBLESHOOTING.................. 69

Minimizing Blank Corrections .............................................69

Operation Away from Ambient Temperature......................69

Stirring Speeds .......................................................................69

1 2 3 4 5 6 7 8 9 10 11 ... 75 76

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